The amino acid sequence around the active-site cysteine and histidine residues of stem bromelain.
نویسندگان
چکیده
Stem bromelain that had been irreversibly inhibited with 1,3-dibromo[2-(14)C]-acetone was reduced with sodium borohydride and carboxymethylated with iodoacetic acid. After digestion with trypsin and alpha-chymotrypsin three radioactive peptides were isolated chromatographically. The amino acid sequences around the cross-linked cysteine and histidine residues were determined and showed a high degree of homology with those around the active-site cysteine and histidine residues of papain and ficin.
منابع مشابه
Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases.
The amino acid sequences of ananain (EC3.4.22.31) and stem bromelain (3.4.22.32), two cysteine proteases from pineapple stem, are similar yet ananain and stem bromelain possess distinct specificities towards synthetic peptide substrates and different reactivities towards the cysteine protease inhibitors E-64 and chicken egg white cystatin. We present here the complete amino acid sequence of ana...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 117 2 شماره
صفحات -
تاریخ انتشار 1970